Estrogen and DNA damage modulate mRNA levels of genes involved in homologous recombination repair in estrogen-deprived cells

Aims: Breast and ovarian cancers are frequently associated with mutations in genes involved DNA double-strand break (DSB) repair by homologous recombination (HR). Risk factors for breast cancer often linked to estrogen-related pathways. Here, we studied the crosslink between estrogen HR pathway. Methods: We analyzed, using online annotation tolls, enrichment of candidate estrogen-upregulated among analyzed how modulates mRNA levels (HRR) estrogen-receptor (ER)-positive cells. The cells were deprived estrogen, HRR determined real-time polymerase chain reaction, following addition as well damage induction. In addition, examined effect on repair, immuno-fluorescence analysis, DSB marker phospho-histone H2AX, an indicator repair. Finally, performed a clonogenic survival assay determine cell survival. Results: discovered that whose upregulated strongly validated upregulates MRE11, RAD50, PALB2, which have not been previously shown be regulated estrogen. Additionally, revealed induces upsurge mRNAs encoding BRCA1, CtIP, ER-positive Notably, was impaired compared exposed hormone. also established hypersensitive DSBs. Conclusion: These results suggest exposure triggers expression genes, is required meet increased demands due proliferating induced This may explain higher chances developing estrogen-dependent genes.